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	Provedor de dados Agronomy (1) Biological Sciences (1) Autor Borges, Wardsson Lustrino (2) Costa Júnior, José de Souza (1) Diédhiou, Abdala Gamby (1) Martins, Claudia Miranda (1) Rumjanek, Norma Gouvêa (1) Sadio, Oumar (1) de Faria, Sergio Miana (1) Mais... Palavra-chave 16S rDNA (1) Activated charcoal (1) Biological nitrogen fixation (1) Ciência do Solo Flooding-tolerant legume (1) Ciências Agrárias (1) DNA quality (1) PCR-RFLP Microbiologia do Solo (1) Pantanal (1) Symbioses (1) TE buffer (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2014 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Assessment of DNA extraction methods for detection of arbuscular mycorrhizal fungi in plant roots by nested-PCR Biological Sciences Diédhiou, Abdala Gamby; Borges, Wardsson Lustrino; Sadio, Oumar; de Faria, Sergio Miana. DNA extraction methods were evaluated for the yield and purity of DNA recovered from mycorrhized roots and whether the recovered DNA is suitable for amplification of arbuscular mycorrhizal (AM) fungal SSU rDNA. The DNeasy Plant Mini Kit and three extraction buffers were used alone or in combination with either polyvinylpyrrolidone (PVP), polyvinylpolypyrrolidone (PVPP) and/or activated charcoal (AC). Among the extraction methods tested, those based on the CTAB buffers yielded more DNA than those based on the TE buffer and the DNeasy Plant Mini Kit. Moreover, the use of AC alone or in combination with PVPP reduced DNA yield, while it significantly improved the purity of recovered DNA, whatever the extraction buffer. On the other hand, the success of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Activated charcoal; TE buffer; DNA quality. Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/21689 Rhizobial diversity from stem and root nodules of Discolobium and Aeschynomene Agronomy Martins, Claudia Miranda; Borges, Wardsson Lustrino; Costa Júnior, José de Souza; Rumjanek, Norma Gouvêa. Among the genera that exhibit stem nodulation, Aeschynomene and Discolobium are found in the Pantanal Mato-grossense (wetlands in the state of Mato Grosso, Brazil). Isolates obtained from the stem and root nodules of D. pulchellum, D. psoraleaefolium, D. leptophyllum and A. fluminensis were collected from various locations in the Pantanal de Poconé sub-region and phenotypically characterized and genotyped by restriction analysis of the 16S rDNA gene. Of the 282 isolates obtained from the stem and root nodules, 84.3% alkalized YMA media and 74.1% showed slow growth. No differences in either the phenotype or the genotype among the rhizobial populations isolated from the root or stem nodules of the species Discolobium and A. fluminensis were observed. Among... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciências Agrárias; Ciência do Solo Flooding-tolerant legume; Symbioses; Pantanal; 16S rDNA; Biological nitrogen fixation; PCR-RFLP Microbiologia do Solo. Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19565 Registros recuperados: 2 Primeira ... 1 ... Última

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